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液相色譜儀中正反相色譜係統置換現象-上海91福利在线观看分析儀器有限公司

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液相色譜儀中正反相色譜係統置換現象

更新時間:2013-05-15點擊次數:6749

在液相色譜分析中關於正反相色譜的置換內容91福利在线观看做以下總結:1.首先用正己烷(矽膠柱)將色譜係統包括正相柱平衡好(保證20分鍾內基線平直,無峰出現);
  
  2.卸掉矽膠柱,封好保存;
  
  3.將進樣閥與檢測器短路,用異丙醇衝洗色譜係統10分鍾(流速1ml/min).在這期間空撥進樣閥三次;
  
  4.換甲醇(或已睛)衝洗係統20分鍾(流速2ml/min),此期間空撥進樣閥三次;
  
  5.安上反相色譜柱,流速調到1ml/min,用甲醇(或已睛)衝洗係統,直到係統平衡;
  
  6.進三針標準品,檢驗麵積﹑保留時間是否重複,若不重複,繼續衝洗直到重複。

  

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